Rat Major Histocompatibility ComplexII (MHCII/AgBII/H-1II) ELISA Kit
Biozol Catalog Number:
BYT-ORB1946965
Supplier Catalog Number:
orb1946965
Alternative Catalog Number:
BYT-ORB1946965-48,BYT-ORB1946965-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
MHCII/AgBII/H-1II
Rat Major Histocompatibility ComplexII (MHCII/AgBII/H-1II) ELISA Kit
Range:
7.82-500ng/mL
Sensitivity:
4.69 ng/mL
Samples:
serum, plasma, Tissue homogenate and Other biological samples
Target:
MHCII/AgBII/H-1II
Application Notes:
Application Notes: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MHCII/AgBII/H-1II. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MHCII/AgBII/H-1II and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MHCII/AgBII/H-1II, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat MHCII/AgBII/H-1II. You can calculate the concentration of Rat MHCII/AgBII/H-1II in the samples by comparing the OD of the samples to the standard curve
orb1946965
orb1946965
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