Rat Signal Transducer and Activator of Transcription 5B (STAT5B) ELISA Kit
Biozol Catalog Number:
BYT-ORB1946949
Supplier Catalog Number:
orb1946949
Alternative Catalog Number:
BYT-ORB1946949-48,BYT-ORB1946949-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
STAT5B, STAT5, signal transducer and activator of transcription 5B
Rat Signal Transducer and Activator of Transcription 5B (STAT5B) ELISA Kit
Range:
0.16-10ng/mL
Sensitivity:
0.1 ng/mL
Samples:
serum, plasma, Tissue homogenate and Other biological samples
Target:
STAT5B
Application Notes:
Application Notes: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat STAT5B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat STAT5B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat STAT5B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat STAT5B. You can calculate the concentration of Rat STAT5B in the samples by comparing the OD of the samples to the standard curve
orb1946949
orb1946949
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