Human 11, 12-dihydroxyeicosatrienoic acids (11, 12-DHETs) ELISA Kit
Biozol Catalog Number:
BYT-ORB1946833
Supplier Catalog Number:
orb1946833
Alternative Catalog Number:
BYT-ORB1946833-48,BYT-ORB1946833-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
11,12-DHETs
Human 11, 12-dihydroxyeicosatrienoic acids (11, 12-DHETs) ELISA Kit
Range:
1.57-100ng/mL
Sensitivity:
0.94 ng/mL
Samples:
serum, plasma, Tissue homogenate and Other biological samples
Target:
11,12-DHETs
Application Notes:
Application Notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human 11,12-DHETs. During the reaction, Human 11,12-DHETs in the sample or standard competes with a fixed amount of Human 11,12-DHETs on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human 11,12-DHETs. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Human 11,12-DHETs in tested samples can be calculated by comparing the OD of the samples to the standard curve
orb1946833
orb1946833
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