Human Trypsinogen Activation Peptide (TAP) ELISA Kit
Biozol Catalog Number:
BYT-ORB1946815
Supplier Catalog Number:
orb1946815
Alternative Catalog Number:
BYT-ORB1946815-48,BYT-ORB1946815-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
TAP
Human Trypsinogen Activation Peptide (TAP) ELISA Kit
Range:
0.25-16pmol/mL
Sensitivity:
0.15 pmol/mL
Samples:
serum, plasma, Tissue homogenate and Other biological samples
Target:
TAP
Application Notes:
Application Notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human TAP. During the reaction, Human TAP in the sample or standard competes with a fixed amount of Human TAP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human TAP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Human TAP in tested samples can be calculated by comparing the OD of the samples to the standard curve
orb1946815
orb1946815
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