Rat Delta Sleep Inducing Peptide (deltaSIP) ELISA Kit

Catalog Number: BYT-ORB1946794
Article Name: Rat Delta Sleep Inducing Peptide (deltaSIP) ELISA Kit
Biozol Catalog Number: BYT-ORB1946794
Supplier Catalog Number: orb1946794
Alternative Catalog Number: BYT-ORB1946794-48,BYT-ORB1946794-96
Manufacturer: Biorbyt
Category: Kits/Assays
Species Reactivity: Rat
Alternative Names: Delta sleep-inducing peptide, DSIP
Rat Delta Sleep Inducing Peptide (deltaSIP) ELISA Kit
Range: 0.16-10ng/mL
Sensitivity: 0.1 ng/mL
Samples: serum, plasma, Tissue homogenate and Other biological samples
Target: deltaSIP
Application Notes: Application Notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat deltaSIP. During the reaction, Rat deltaSIP in the sample or standard competes with a fixed amount of Rat deltaSIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat deltaSIP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Rat deltaSIP in tested samples can be calculated by comparing the OD of the samples to the standard curve
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