Serum, Plasma, Tissue homogenate and Other biological samples
Target:
LPS
Application Notes:
Application Notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LPS. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to LPS. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of LPS in the samples is then determined by comparing the OD of the samples to the standard curve
orb1946412
orb1946412
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