Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application Dilute:
WB - 1:2000
ATP7B Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of ATP7B Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ATP7B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Anti-ATP7B Antibody (C-term) at 1:2000 dilution + Rat liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 157 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-ATP7B Antibody (C-term) at 1:2000 dilution + Mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 157 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-ATP7B Antibody (C-term) at 1:2000 dilution + Caco2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 157 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: ATP7B Antibody (C-term) at 1:2000 dilution. Lane 1: Caco2 whole cell lysate. Lane 2: Mouse kidney lysate. Lane 3: Rat liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 157 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
* VAT and and shipping costs not included. Errors and price changes excepted
