Phospho-Tau (Thr181) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB1878
- Images (6)
| Article Name: | Phospho-Tau (Thr181) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB1878 |
| Supplier Catalog Number: | orb1878 |
| Alternative Catalog Number: | BYT-ORB1878-50,BYT-ORB1878-100,BYT-ORB1878-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Thr181 PK(p-T)PP |
| Conjugation: | Unconjugated |
| Alternative Names: | MAPT | Tau (p-T181), p-Tau, phospho-Tau, tau, DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, Tau-PHF6, tau-40, Mtapt, PHF-tau, MAPT_0N4R, MAPT_1N4R, MAPT_2N4R, RNPTAU, pTau, TAU_BOVIN, MAPT, Neurofibrillary tangle protein, Paired helical filament-tau (PHF-tau), TAU_HUMAN, TAU_MOUSE, TAU_RAT, |
| Phospho-Tau (Thr181) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 52/83 kDa |
| UniProt: | P10636 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | MAPT |
| Application Dilute: | WB=1:500-2000 |
| Application Notes: | Modification: Phosphorylated |
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Blank control (blue line): MCF 7 (fixed with 70% methanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-Tau (Thr181) antibody (orb1878), dilution: 3 µg/10 5 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. |
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Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Tau (Thr181)) Polyclonal Antibody, Unconjugated (orb1878) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Tau (Thr181)) Polyclonal Antibody, Unconjugated (orb1878) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Lane 3: Cerebellum (Mouse) Lysate at 40 ug, Lane 4: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-phospho-Tau (Thr181) (orb1878) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-70 kD, Observed band size: 50 kD. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-Tau (Thr181) Polyclonal Antibody, Unconjugated (orb1878) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (phospho-Tau (Thr181)) polyclonal Antibody, Unconjugated (orb1878) 1:100, 90 minutes at 37C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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