Beta Defensin 3 Rabbit Polyclonal Antibody (FITC)

Catalog Number: BYT-ORB187581
Article Name: Beta Defensin 3 Rabbit Polyclonal Antibody (FITC)
Biozol Catalog Number: BYT-ORB187581
Supplier Catalog Number: orb187581
Alternative Catalog Number: BYT-ORB187581-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC
Species Reactivity: Human
Immunogen: KLH conjugated synthetic peptide derived from human beta Defensin 3 (23-67/67aa)
Conjugation: FITC
Alternative Names: BD 3, BD-3, BD3, beta 103, beta defensin 103A, beta defensin 103A precursor, beta defensin 3, beta-defensin 103, beta-defensin 3, D103A_HUMAN, DEF B3, DEFB 103, DEFB 103A, DEFB 3, DEFB-3, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin, Defensin beta 103A, Defensin beta 3, Defensin like protein, Defensin-like protein, HBD 3, hBD-3, HBD3, HBP 3, HBP3, mBD3.
Beta Defensin 3 Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting DEFB103A. This antibody is suitable for FC. It exhibits reactivity with Human samples.
Clonality: Polyclonal
Concentration: 1mg/ml
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Target: DEFB103A
Application Dilute: Flow-Cyt=1µg/Test
Blank control (blue): Hep G2 Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-beta Defensin 3/FITC Conjugated antibody (orb187581), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.
Paraformaldehyde-fixed, paraffin embedded (Human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation: Anti-beta Defensin 3 Antibody, conjugated (orb187581) 1:400, 1.5 hours at 37C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation: Anti-beta Defensin 3 Antibody, conjugated (orb187581) 1:400, 1.5 hours at 37C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
Immunofluorescence analysis of Human kidney using beta Defensin 3 antibody (FITC).
Immunofluorescence analysis of Human colon using beta Defensin 3 antibody (FITC).
Flow cytometric analysis of HepG2 Cells using beta Defensin 3 antibody (FITC).