VPS35/MEM3 Antibody, Unconjugated, Goat, Polyclonal

Catalog Number: BYT-ORB18631
Article Name: VPS35/MEM3 Antibody, Unconjugated, Goat, Polyclonal
Biozol Catalog Number: BYT-ORB18631
Supplier Catalog Number: orb18631
Alternative Catalog Number: BYT-ORB18631-100
Manufacturer: Biorbyt
Host: Goat
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Alternative Names: anti VPS35 antibody, anti MEM3 antibody, anti FLJ10752 antibody, anti FLJ13588 antibody, anti FLJ20388 antibody, anti DKFZp434E1211 antibody, anti DKFZp434P1672 antibody, anti vacuolar protein sorting 35 (yeast) antibody, anti maternal-embryonic 3 antibody, anti vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, anti vacuolar protein sorting 35 antibody
Goat polyclonal antibody to VPS35
Clonality: Polyclonal
Concentration: 1-3 µg/ml
Molecular Weight: 91.7
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence: SPESEGPIYEGLIL
Target: VPS35 / MEM3
Application Dilute: ELISA: 1:128000, WB: 1.0-3.0 µg/ml, IHC-P: 5-8 µg/ml
Application Notes: Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.WB: Approx 90kDa band observed in Human Brain and Testis lysates (predicted MW of 91kDa according to NP_060676.2). Recommended for use at 1-3 µg/ml.Experiment Notes: Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please see Scott KL et al., Nature. 2009 Jun 25,459(7250):1085-90., PMID: 19553991
Western blot analysis of Human (A) Mouse (B) and Rat (C) Brain lysate using VPS35 antibody.
Western blot analysis of HepG2 (A) and HEK293 (B) cell lysate using VPS35 antibody.
Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells using VPS35 antibody.
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using VPS35 antibody.
Immunohistochemical staining of Human Prostate using VPS35 antibody
Immunohistochemical staining of Human Prostate using VPS35 antibody