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PI3 Kinase p110 beta Rabbit Polyclonal Antibody, Unconjugated

PI3 Kinase p110 beta Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB185885

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Article Name:	PI3 Kinase p110 beta Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number:	BYT-ORB185885
Supplier Catalog Number:	orb185885
Alternative Catalog Number:	BYT-ORB185885-50,BYT-ORB185885-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	KLH conjugated synthetic peptide derived from human PI3 Kinase p110 beta
Conjugation:	Unconjugated
Alternative Names:	P110BETA, PI3K, PI3KBETA, PIK3C1, 1110001J02Rik, PK3CB_HUMAN, PIK3CB, PI3-kinase subunit beta, PI3K-beta, PtdIns-3-kinase subunit beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta (PtdIns-3-kinase subunit p110-beta \| p110beta), Serine/threonine protein kinase PIK3CB, 2.7.1.153, Q24JU2_HUMAN, Q3U4Q1_MOUSE, PK3CB_MOUSE, PK3CB_RAT,

PI3 Kinase p110 beta Rabbit Polyclonal Antibody

Clonality:	Polyclonal
Concentration:	1mg/ml
Molecular Weight:	123 kDa
UniProt:	P42338
Buffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:	Liquid
Target:	PIK3CB

Application Dilute:

WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500

	Blank control: A549. Primary Antibody (green line): Rabbit Anti-PI3 Kinase p110 beta antibody (orb185885), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
	Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PI3 Kinase p110 beta) Polyclonal Antibody, Unconjugated (orb185885) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PI3 Kinase p110 beta) Polyclonal Antibody, Unconjugated (orb185885) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Sample: Jurkat (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-PI3 Kinase p110 beta (orb185885) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 123 kD, Observed band size: 123 kD.
	Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-PI3 Kinase p110 beta (orb185885) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 123 kD, Observed band size: 123 kD.
	Western blot:Placenta (Mouse),Primary:Anti-PI3 Kinase p110 beta at 1:1000 dilution.

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