MYL2 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB185379
- Images (6)
| Article Name: | MYL2 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB185379 |
| Supplier Catalog Number: | orb185379 |
| Alternative Catalog Number: | BYT-ORB185379-50,BYT-ORB185379-100,BYT-ORB185379-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Cardiac ventricular myosin light chain 2 (11-110/166aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | MLC-2s/v, CMH10, MFM12, MLC-2, MLC-2v, MLC2, Gm32672, Mlc2v, Mylpc, MLRV_BOVIN, MYL2, Myosin light chain 2, slow skeletal/ventricular muscle isoform (MLC-2s/v), MLRV_HUMAN, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLRV_MOUSE, MLRV_RAT, |
| MYL2 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 20 kDa |
| UniProt: | P10916 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | MYL2 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
|
|
Paraformaldehyde-fixed, paraffin embedded (Human heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYL2) Polyclonal Antibody, Unconjugated (orb185379) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYL2) Polyclonal Antibody, Unconjugated (orb185379) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYL2) Polyclonal Antibody, Unconjugated (orb185379) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYL2) Polyclonal Antibody, Unconjugated (orb185379) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYL2) Polyclonal Antibody, Unconjugated (orb185379) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-MYL2 (orb185379) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD. |
Product Guarantee and Expert Support
