anti IRF5 antibody, anti interferon regulatory factor 5 antibody, anti SLEB10 antibody
Goat polyclonal antibody to IRF5
Clonality:
Polyclonal
Molecular Weight:
56.0, 56.0, 57.8
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Sequence:
QGPWPMHPAGMQ
Target:
IRF5
Application Dilute:
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 55+60kDa bands observed in Human Spleen lysates, approx. 65kDa in lysates of cell line A549, and approx. 70kDa in lysates of cell lines Daudi (calculated MW of 57.8kDa accord
Western blot analysis of Human Spleen (Lane 1) and Peripheral Blood Lymphocytes (Lane 2) lysates using IRF5 antibody
Western blot analysis of HEK293 lysate using IRF5 antibody
Primary incubation 1 hour at room temperature. Image A: Human Spleen lysate at primary Ab concentration 1 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
5 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Primary incubation 1 hour at room temperature. Images A+B: A549 cell lysate + peptide, incubation at primary Ab concentration 1 ug/ml and C+D: Daudi cell lysate + peptide, incubation at primary Ab concentration 1 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
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