GLIS3 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB183858
- Images (6)
| Article Name: | GLIS3 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB183858 |
| Supplier Catalog Number: | orb183858 |
| Alternative Catalog Number: | BYT-ORB183858-50,BYT-ORB183858-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Rat, Sheep |
| Immunogen: | KLH conjugated synthetic peptide derived from human GLIS3 (351-450/775aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | NDH, ZNF515, GLIS3_HUMAN, GLIS3, GLI-similar 3, Zinc finger protein 515, GLIS3_MOUSE, |
| GLIS3 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 84 kDa |
| UniProt: | Q8NEA6 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | GLIS3 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
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Sample: Lane 1: Human U251 cell lysates, Lane 2: Human U87MG cell lysates, Lane 3: Human A549 cell lysates, Lane 4: Human 293T cell lysates, Lane 5: Human MDA-MB-231 cell lysates, Primary: Anti-GLIS3 (orb183858) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 84 kDa, Observed band size: 84 kDa. |
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Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (orb183858) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (orb183858) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: Rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (orb183858) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat thyroid gland, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (orb183858) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: Sheep thyroid tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (orb183858) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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