FITM1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB183695
Article Name: FITM1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB183695
Supplier Catalog Number: orb183695
Alternative Catalog Number: BYT-ORB183695-50,BYT-ORB183695-100,BYT-ORB183695-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Rat
Immunogen: KLH conjugated synthetic peptide derived from human FITM1 (1-100/292aa)
Conjugation: Unconjugated
Alternative Names: FIT1, 1110028A07Rik, Cg10671, FITM1_BOVIN, FITM1, Fat-inducing protein 1, FITM1_HUMAN, FITM1_MOUSE, FITM1_PIG,
FITM1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 32 kDa
UniProt: A5D6W6
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: FITM1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of Rat stomach tissue using FITM1 antibody.
Immunohistochemical staining of rat liver tissue using FITM1 antibody.
Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Fat inducing transcript, FITM1) Polyclonal Antibody, Unconjugated (orb183695) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FITM1) Polyclonal Antibody, Unconjugated (orb183695) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Fat inducing transcript, FITM1) Polyclonal Antibody, Unconjugated (orb183695) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Protein: 293T (human) lysate at 40 ug, Primary: rabbit Anti-FITM1 (orb183695) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 32 kD, Observed band size: 28 kD.



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