ENT2 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB183481
Article Name: ENT2 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB183481
Supplier Catalog Number: orb183481
Alternative Catalog Number: BYT-ORB183481-50,BYT-ORB183481-100,BYT-ORB183481-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human ENT2 (221-320/456aa)
Conjugation: Unconjugated
Alternative Names: DER12, ENT2, HNP36, hENT2, mENT2, rENT2, S29A2_HUMAN, SLC29A2, 36 kDa nucleolar protein HNP36, Delayed-early response protein 12, Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter (Equilibrative NBMPR-insensitive nucleoside transporter), Hydrophobic nucleolar protein, 36 kDa, Nucleoside transporter, ei-type, Solute carrier family 29 member 2, S29A2_MOUSE, 36 kDa hydrophobic nucleolar protein, Q9JIT8_MOUSE, S29A2_RAT,
ENT2 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 50 kDa
UniProt: Q14542
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: SLC29A2
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat brain tissue using ENT2 antibody.
Western blot analysis of mouse heart Cell lysate using ENT2 antibody.
Protein: brain (mouse) lysate at 40 ug, Primary: rabbit Anti-ENT2 (orb183481) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 50 kD, Observed band size: 55 kD.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Muscle (Mouse) Lysate at 40 ug, Liver (Mouse) Lysate at 40 ug, Primary: Anti-ENT2 (orb183481) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-ENT2 Polyclonal Antibody, Unconjugated (orb183481) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-ENT2 Polyclonal Antibody, Unconjugated (orb183481) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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