ELP4 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB183376
Article Name: ELP4 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB183376
Supplier Catalog Number: orb183376
Alternative Catalog Number: BYT-ORB183376-50,BYT-ORB183376-100,BYT-ORB183376-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human ELP4 (321-424/424aa)
Conjugation: Unconjugated
Alternative Names: ELP4_BOVIN, ELP4, ELP4_HUMAN, hELP4, PAX6 neighbor gene protein, C11orf19, PAXNEB, ELP4_MOUSE, ELP4_DANRE,
ELP4 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 47 kDa
UniProt: Q96EB1
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: ELP4
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Western blot analysis of Hela lysate using ELP4 antibody.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ELP4) Polyclonal Antibody, Unconjugated (orb183376) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ELP4) Polyclonal Antibody, Unconjugated (orb183376) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ELP4) Polyclonal Antibody, Unconjugated (orb183376) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Protein: Hela (human) lysate at 40 ug, Primary: rabbit Anti-ELP4 (orb183376) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 47 kD, Observed band size: 47 kD.
Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Prostate tissue lysates, Lane 3: Rat Stomach tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-ELP4 (orb183376) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 50 kDa.



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