Quantitative colorimetric detection of creatinine in urine samples
Range:
0.3125- 20 mg/dl
Sensitivity:
0.032 mg/dl
Samples:
Urine
Target:
Urine Creatinine Detection Kit
Application Notes:
Application Notes: The Urine Creatinine Detection kit is designed to quantitatively measure creatinine present in urine samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate. The color generating reaction is initiated with the StressXpress Creatinine Reagent, which is pipetted into each well. After a short incubation the intensity of the generated color is detected in a microtiter plate reader capable of measuring 490nm wavelength. The concentration of the creatine in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. The Jaffe reaction used in this kit has been modified to read creatinine levels in urine(7,8)
Typical Standard Curve for the Urine Creatinine Detection Kit Assay Type: Quantitative. Detection Method: Colorimetric Assay. Assay Range: 0.3125- 20 mg/dl.
Conversion of creatine and p-creatine to creatinine. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from p-creatine.
Linearity was determined by taking two diluted 1:20 human urine samples, one with a low diluted creatinine level of 0.38 mg/dL and one with a higher diluted level of 9.33 mg/dL and mixing them in the ratios given below. The measured concentrations were compared to the expected values.
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