KLH conjugated synthetic peptide derived from human XIAP (201-330/496aa)
Conjugation:
FITC
Alternative Names:
X-linked Inhibitor of Apoptosis Protein, RIAP-3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, Inhibitor of apoptosis protein 3, X-linked IAP, IAP-like protein, HILP, BIRC4, HILP, XLP2, ILP1, Xiap, MIHA, hILP, IAP3, API3, XIAP, XIAP_HUMAN, ILP, hILP, IAP-3, hIAP-3, hIAP3, X-linked inhibitor of apoptosis protein.
XIAP/BIRC4 Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting XIAP. This antibody is suitable for FC, ICC, IF. It exhibits reactivity with Human, Mouse, Rat samples.
Clonality:
Polyclonal
Concentration:
1mg/ml
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Target:
XIAP
Application Dilute:
Flow-Cyt=3ug/Test, IF=1:100-500, ICC/IF=1:100-500
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-BIRC4 antibody (orb16506), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-XIAP/BIRC4 antibody (orb16506), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-XIAP/BIRC4 antibody (orb16506), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Flow cytometric analysis of Molt4 cell lysate using XIAP antibody (FITC)
Flow cytometric analysis of Hela cell lysate using XIAP antibody (FITC)
Flow cytometric analysis of MCF7 cell lysate using XIAP antibody (FITC)
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