TRPV4 agonist-1 free base (OUN67600) is a potent and selective small molecule agonist of the TRPV4 ion channel, demonstrating an EC50 of 60 nM in a human TRPV4 calcium influx assay. This compound is a valuable research tool for investigating TRPV4-mediated signaling in various physiological and pathological contexts, including studies of osmoregulation, pain, and inflammation in both cellular and animal models.