Synthetic peptide / encompassing a sequence within the N-terminus region.
Conjugation:
Unconjugated
Rabbit polyclonal antibody to Nestin antibody (NES)
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
Nestin / NES
Application Dilute:
Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:200
Anti-Nestin antibody at 1/1000 dilution, Lysates at 30 µg per lane.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294409 anti-Nestin antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.