Synthetic peptide / Extracellular domain corresponding to Human CD133.
Conjugation:
Unconjugated
Rabbit polyclonal antibody to CD133
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
CD133 / Prominin-1 (Intracellular domain)
Application Dilute:
Western Blot 1:500-1:1000, Immunofluorescence 1:300-1:500, Immunohistochemistry (Paraffin) 1:200-1:400
All lanes: Anti-CD133 antibody at 1/700 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294396 overnight at 4C.
All lanes: Anti-CD133 antibody at 1/700 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294396 overnight at 4C.
All lanes: Anti-CD133 antibody at 1/700 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294396 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294396 anti-CD133 / Prominin-1 antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD133 / Prominin-1 with orb1294396 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD133 / Prominin-1 with orb1294396 at 1/200.
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