Synthetic peptide corresponding to residues surrounding Thr181 of human Tau protein
Conjugation:
Unconjugated
Rabbit polyclonal antibody to Tau (Phospho Thr181)
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 30% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
Phospho-Tau (Thr181)
Application Dilute:
Western Blot 1:500-1:1500, Immunofluorescence 1:200-1:400, Immunohistochemistry (Paraffin) 1:100
Application Notes:
Modification: Phosphorylated
Anti-Phospho-Tau (Thr181) antibody at 1/1000 dilution, Lysates/proteins at 55 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294393 overnight at 4C.
Anti-Phospho-Tau (Thr181) antibody at 1/1000 dilution, Lysates/proteins at 55 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294393 overnight at 4C.
Immunofluorescent analysis. Sample: cortical neurons, Phospho-Tau (Thr181) antibody (orb1294393): 1-200, Red: MAP2 antibody, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.
Immunohistochemical analysis of paraffin embedded Human tissue labeling phosphor-Tau (Thr181) with orb1294393 at 1/100.
