Synthetic peptide / encompassing a sequence within the C-terminus region.
Conjugation:
Unconjugated
Rabbit polyclonal antibody to IL1 beta
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine,1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
IL-1 beta
Application Dilute:
Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:300
All lanes: Anti-IL1 beta antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294342 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294342 anti-IL1 beta antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling IL1 beta with orb1294342 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling IL1 beta with orb1294342 at 1/200.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling IL1 beta with orb1294342 at 1/200.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling IL1 beta with orb1294342 at 1/200.
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