Synthetic peptide / encompassing a sequence within the center region
Conjugation:
Unconjugated
Rabbit polyclonal antibody to p21 Cip1
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
p21 Waf1 / Cip1
Application Dilute:
Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:400, Immunohistochemistry (Paraffin) 1:100-1:200
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294337 anti-p21 Cip1 antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p21 Cip1 with orb1294337 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p21 Cip1 with orb1294337 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p21 Cip1 with orb1294337 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling p21 Cip1 with orb1294337 at 1/100.
p21 Cip1 antibody at 1/500 dilution. Lysates at 30 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294337 overnight at 4C.
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