Synthetic peptide / encompassing a sequence within the center region
Conjugation:
Unconjugated
Rabbit polyclonal antibody to ATF6
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 1%rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
ATF6
Application Dilute:
Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:300, Immunohistochemistry (Paraffin) 1:200-1:400
All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4C.
All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4C.
All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4C.
All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294330 anti-ATF6 antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ATF6 with orb1294330 at 1/200.
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