Synthetic peptide / encompassing a sequence within the center region
Conjugation:
Unconjugated
Rabbit polyclonal antibody to PERK
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
PERK / EIF2AK3
Application Dilute:
Western Blot 1:500, Immunofluorescence 1:300-1:500, Immunohistochemistry (Paraffin) 1:100-1:200
All lanes: Anti-PERK antibody - at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294328 overnight at 4C.
All lanes: Anti-PERK antibody - at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294328 overnight at 4C.
All lanes: Anti-PERK antibody - at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294328 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294328 anti-PERK antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PERK with orb1294328 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PERK with orb1294328 at 1/200.
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