Synthetic peptide / Extracellular domain corresponding to Human CD81
Conjugation:
Unconjugated
Rabbit polyclonal antibody to CD81
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
CD81
Application Dilute:
Western Blot 1:500, Immunohistochemistry (Paraffin) 1:100 - 1:200, Flow Cytometry 1:50 - 1:100
Anti-CD81 antibody at 1/500 dilution, Lysates at 40 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294247 overnight at 4C.
Anti-CD81 antibody at 1/500 dilution, Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294247 overnight at 4C.
Anti-CD81 antibody at 1/500 dilution, Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294247 overnight at 4C.
Anti-CD81 antibody at 1/500 dilution, Lysates/proteins at 65 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294247 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294247 anti-CD81 antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD81 with orb1294247 at 1/100.
* VAT and and shipping costs not included. Errors and price changes excepted
