Recombinant human Mesothelin(37-286) protein with C-terminal 6*His tag
Molecular Weight:
The protein has a predicted molecular mass of 27.6 kDa after removal of the signal peptide. The apparent molecular mass of Mesothelin(37-286)-His is approximately 25-35 kDa due to glycosylation.
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Purity:
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Form:
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Target:
Mesothelin
SARS-CoV-2 (2019-nCoV) S protein RBD (N501Y), hFc Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 µg/mL (100 µL/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag (orb1173916) can bind Human ACE2 Protein, mFc Tag orb689420 in a linear range of 4.115-1000 ng/ml.
ELISA plate pre-coated by 2 µg/mL (100 µL/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag (orb1173916) can bind Anti-SARS-CoV-2 (CB6 biosimilar) mAb orb689316 in a linear range of 1.00-97.66 ng/ml.
ELISA plate pre-coated by 2 µg/mL (100 µL/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag (orb1173916) can bind Anti-SARS-CoV-2 RBD antibody, Rabbit mAb orb757389 in a linear range of 0.244-15.625 ng/ml.
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