DNAJB6 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256633
Article Name: DNAJB6 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1256633
Supplier Catalog Number: orb1256633
Alternative Catalog Number: BYT-ORB1256633-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).
Conjugation: Unconjugated
Alternative Names: DNAJB6, DKFZp566D0824, DnaJ, FLJ42837, HHDJ1, HSJ-2, HSJ2, MGC1152, MGC117297, MRJ, MSJ-1, DJ4, LGMD1D, LGMD1E
DNAJB6 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 40kDa
UniProt: O75190
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: DNAJB6
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using DNAJB6 antibody (orb1256633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DNAJB6 antibody (orb1256633). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using DNAJB6 antibody (orb1256633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.