TIGIT Antibody, IgG2b, Clone: [7E5], Unconjugated, Mouse, Monoclonal

Catalog Number: BYT-ORB1240147
Article Name: TIGIT Antibody, IgG2b, Clone: [7E5], Unconjugated, Mouse, Monoclonal
Biozol Catalog Number: BYT-ORB1240147
Supplier Catalog Number: orb1240147
Alternative Catalog Number: BYT-ORB1240147-0.02,BYT-ORB1240147-0.1
Manufacturer: Biorbyt
Host: Mouse
Category: Antikörper
Application: ELISA, FC, ICC, IF, IHC-P
Species Reactivity: Human
Immunogen: TIGIT antibody was raised against the extracellular domain of human TIGIT.
Conjugation: Unconjugated
Alternative Names: TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
TIGIT Antibody
Clonality: Monoclonal
Concentration: 1 mg/mL
Clone Designation: [7E5]
Molecular Weight: Predicted: 26 kDa
Isotype: IgG2b
NCBI: 776160
UniProt: Q495A1
Buffer: TIGIT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Form: Liquid
Target: TIGIT
Application Notes: Application Notes: TIGIT antibody can be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 20 µg/mL.Antibody validated: Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested
Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 µg/ml.
Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 µg/ml. Green: TIGIT Antibody [7E5] (orb1240147) Blue: DAPI staining.
Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 µg/ml. Green: TIGIT Antibody [7E5] (orb1240147) Blue: DAPI staining
Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 µg/ml.
Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 µg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.
Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with orb1240147, orb1240136, orb1240137, orb1240140, orb1240146 and orb1240115 antibodies at decreasing concentrations.