Anti-PKR antibody (orb1239891) was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR.
Conjugation:
Unconjugated
Alternative Names:
PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2
PKR Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
EIF2AK2
Western Blot Validation of PKR in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PKR orb1239891 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PKR orb1239891 (1 µg/mL), PKR orb1238780 (1 µg/mL, and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of PKR in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with orb1239891 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunohistochemistry Validation of PKR in Rat Lung. Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (orb1239891) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of PKR in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with orb1239891 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunohistochemistry Validation of PKR in Mouse Lung. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-PKR antibody (orb1239891) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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