Anti-op18 antibody (orb1239690) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human op18. The immunogen is located within the first 50 amino acids of op18.
op18 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
STMN1
Western Blot Validation in Human Brain Tissue. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239690 (A: 0.5 µg/mL, B: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239686 (2 µg/mL), op18 orb1239690 (2 µg/mL), and GAPDH orb1239765 (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239690 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: op18 orb1239690 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of op18 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-op18 antibody (orb1239690) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of op18 in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling op18 with orb1239690 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
* VAT and and shipping costs not included. Errors and price changes excepted
