Anti-MPYS antibody (orb1239632) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human MPYS. The immunogen is located within amino acids 260-310 of MPYS.
MPYS Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
TMEM173
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (1 µg/mL), MPYS orb1271001 (2 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human THP-1 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse A-20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry Validation of MPYS in Mouse A20 Cells. Immunocytochemical analysis of A20 cells using anti-MPYS antibody (orb1239632) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of MPYS in Moue A20 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling MPYS with orb1239632 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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