Anti-LSD1 antibody (orb1239604) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human LSD1. The immunogen is located within amino acids 40-90 of LSD1.
LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
KDM1
Western Blot Validation in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: LSD1 orb1239604 (0.2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: LSD1 orb1239604 (0.2 µg/mL), LSD1 orb1274534 (1 µg/mL), beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: LSD1 orb1239604 (0.2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse P815 Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: LSD1 orb1239604, (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of LSD1 in Human Breast Carcinoma Tissue. Immunohistochemical analysis of paraffin-embedded human breast carcinoma Tissue using anti-LSD1 antibody (orb1239604) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of LSD1 in human breast carcinoma Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast carcinoma Tissue labeling LSD1 with orb1239604 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green/blue).
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