Anti-LIF antibody (orb1239553) was raised against a peptide corresponding to 16 amino acids near the center of human LIF. The immunogen is located within amino acids 50-100 of LIF.
LIF Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
LIF
Western Blot Validation in Mouse 3T3 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1: 1 µg/mL, 2: 2 µg/mL, 3: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Cell Lines and Tissue. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Lung. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1: 1 µg/mL, 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of LIF in Mouse 3T3 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with orb1239553 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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