Anti-JMJD3 antibody (orb1239526) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human JMJD3. The immunogen is located within amino acids 1370-1420 of JMJD3.
JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
KDM6B
Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: JMJD3 orb1239526 (1 µg/mL), JMJD3 orb1260760, (2 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526 (0.25 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in K562 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide.
Western Blot Validation in Mouse A-20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide.
Western Blot Validation in HepG2 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD3, orb1239526, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 µg/mL, Lane 2: 0.5 µg/mL Lane 3: 0.25 µg/mL with blocking peptide.
Immunocytochemistry Validation of JMJD3 in K562 Cells. Immunocytochemical analysis of K562 cells using anti-JMJD3 antibody (orb1239526) at 2.5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of JMJD3 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling JMJD3 with orb1239526 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
* VAT and and shipping costs not included. Errors and price changes excepted
