Anti-IKK epsilon antibody (orb1239458) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IKK epsilon. The immunogen is located within the last 50 amino acids of IKK epsilon.
IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
IKBKE
Induction Validation in Mouse Cell Line. Loading: 15 µg of Raw264.7 cell lysates per lane. Antibodies: IKK epsilon orb1239458 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Cells were treated with LPS (0.3 µg/mL) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).
Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IKK epsilon orb1239458 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: IKK epsilon orb1239458 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Jurkat Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IKK epsilon orb1239458 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of IKK epsilon in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IKK epsilon antibody (orb1239458) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of IKK epsilon in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling IKK epsilon with orb1239458 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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