NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.The immunogen is located within amino acids 130 - 180 of NANOG.
Conjugation:
Unconjugated
Alternative Names:
NANOG Antibody: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog
NANOG Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
NANOG
Application Notes:
Application Notes: WB: 1-2 µg/mL, ICC/IF: 20 µg/mL, IHC: 1-5 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human, mouse and rat samples and Immunofluorescence in human samples. All other applications and species not yet tested
Immunoflouorescence Validation of NANOG in HeLa Cells. Immunofluorescent analysis of PFA-fixed HeLa cells labeling NANOG with orb1239398 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
WB Validation in HeLa Cells. Loading: 15 µg of lysate. Antibodies: NANOG orb1239398, 2 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse and Rat Testis. Loading: 15 µg of lysates per lane. Antibodies: NANOG orb1239398, 2 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of NANOG in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-NANOG antibody (orb1239398) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of NANOG in Mouse Testis. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NANOG antibody (orb1239398) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of NANOG in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NANOG antibody (orb1239398) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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