Anti-CTRP3 antibody (orb1239313) was raised against a peptide corresponding to 16 amino acids near the center of human CTRP3. The immunogen is located within amino acids 90-140 of CTRP3.
CTRP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
C1QTNF3
Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CTRP3, orb1239313 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 30 ng of human CTRP3 recombinant protein per lane. Antibodies: CTRP3, orb1239313 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse and Rat Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: CTRP3, orb1239313 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Heart Tissue Lysate in the (A) absence and (B) presence of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: CTRP3, orb1239313 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of CTRP3 in Mouse Heart Tissue. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CTRP3 antibody (orb1239313) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of CTRP3 in Mouse Heart Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling CTRP3 with orb1239313 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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