CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
CD4
Application Notes:
Application Notes: WB: 0.5-4 µg/mL, IHC: 5 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples. All other applications and species not yet tested
Western Blot Validation with Human Recombinant Protein.Loading: 5ng (Lane 1), 25ng (Lane 2) and 100ng (Lane 3) of human CD4 recombinant protein. Antibodies: CD4 orb1239267 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Observed at around 27kD.
Western Blot Validation in Human Thymus Tissue Lysate.Loading: 15 µg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Human Jurkat Lysate.Loading: 15 µg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 1 µg/mL, Lane 2: 2 µg/mL and Lane 3: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Human, Mouse and Rat Tissue Lysate.Loading: 15 µg of lysates per lane. Antibodies: CD4 orb1239267 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Human Cell Lines.Loading: 15 µg of lysates per lane. Antibodies: CD4 orb1239267 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Mouse Spleen Tissue Lysate.Loading: 15 µg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 1 µg/mL, Lane 2: 2 µg/mL and Lane 3: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Rat Lung Tissue Lysate.Loading: 15 µg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Immunohistochemistry Validation of CD4 in Human Thymus Tissue.Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (orb1239267) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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