Application Notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC.
Immobilized Human PD-L1, Fc Tag (HPLC-verified) at 10 ug/ml (100 uL/well) can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) with a linear range of 2-40 ng/mL.
Immobilized Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) at 2 ug/ml (100 uL/well) can bind Human PD-L2, Fc Tag with a linear range of 0.2-4 ng/mL.
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