LAG3 Detection Set (Risk Free), Unconjugated, Mouse

Catalog Number: BYT-ORB1227438
Article Name: LAG3 Detection Set (Risk Free), Unconjugated, Mouse
Biozol Catalog Number: BYT-ORB1227438
Supplier Catalog Number: orb1227438
Alternative Catalog Number: BYT-ORB1227438-1
Manufacturer: Biorbyt
Host: Mouse
Category: Antikörper
Application: ELISA, FC, ICC, IF, IHC-P, WB
Species Reactivity: Human
Immunogen: LAG3 antibodies were raised against the extracellular domain of human LAG3.
Conjugation: Unconjugated
LAG3 Detection Set (Risk Free)
Concentration: Antibody 1 mg/mL
Buffer: PBS containing 0.02% sodium azide.
Form: Liquid
Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with orb1239547, orb1239545, orb1239551, orb1239549, orb1239550, orb1239548, and orb1239546 antibodies at decreasing concentrations.
Western blot analysis of LAG-3 in over expressing HEK293 cells using orb1239547, orb1239548, and orb1239546 antibodies at (A) 0.25 (B) 0.5 and (C) 1 µg/ml.
Immunocytochemistry of LAG-3 in over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 1 µg/ml.
Immunofluorescence of LAG-3 in over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 2 µg/ml.
Immunofluorescence of LAG-3 in human spleen tissue using (A) orb1239547, (B) orb1239551, (C) orb1239549, (D) orb1239550, (E) orb1239548, (F) orb1239546, and (G) control mouse IgG antibody at 10 µg/ml.
Immunohistochemistry of LAG-3 in human lymphoma tissue using (A) orb1239547, (B) orb1239551, (C) orb1239549, (D) orb1239550, (E) orb1239548, (F) orb1239546, and (G) control mouse IgG antibody at 5 µg/ml.
Flow cytometry analysis of LAG-3 over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 1 µg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
A sandwich ELISA was performed using the anti-LAG3 mAbs orb1239547, orb1239545, orb1239551, orb1239549, orb1239550 and orb1239548 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.