MFN1 Recombinant Mouse Monoclonal Antibody, Clone: [G4A3], Unconjugated

Catalog Number: BYT-ORB1172516
Article Name: MFN1 Recombinant Mouse Monoclonal Antibody, Clone: [G4A3], Unconjugated
Biozol Catalog Number: BYT-ORB1172516
Supplier Catalog Number: orb1172516
Alternative Catalog Number: BYT-ORB1172516-25,BYT-ORB1172516-50,BYT-ORB1172516-100
Manufacturer: Biorbyt
Host: Mouse
Category: Antikörper
Application: ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Alternative Names: hfzo1, hfzo2, 2310002F04Rik, 6330416C07Rik, D3Ertd265e, HR2, mKIAA4032, Fzo1b, MFN1_HUMAN, MFN1, Fzo homolog, Transmembrane GTPase MFN1, 3.6.5.-, MFN1_MOUSE, MFN1_RAT, Mitochondrial transmembrane GTPase FZO1B,
MFN1 Recombinant Mouse Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Clone Designation: [G4A3]
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MFN1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4C overnight, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HepG2, PC-3, PC-12, EL4.IL-12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 84 kDa, Observed MW: 84 kDa.
Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.