Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.
Clonality:
Monoclonal
Clone Designation:
[M375]
Isotype:
IgG2b
Buffer:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Source:
Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.
Target:
Anti-Phosphotyrosine
Application Dilute:
WB(1:300-5000), IP(1-2ug)
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