Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex)

Catalog Number: BOB-MEK2007
Article Name: Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex)
Biozol Catalog Number: BOB-MEK2007
Supplier Catalog Number: MEK2007
Alternative Catalog Number: BOB-MEK2007-1KIT
Manufacturer: Boster Bio
Category: Kits/Assays

Product Overview

The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 4.83 hours.The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Assay Ranges

AnalyteAssay TypeCalibrator RangeUpper Limit of Quantification (ULOQ)Lower Limit of Quantification (LLOQ)Limit of DetectionPrecision (Inter-assay)Precision (Intra-assay)Average LinearityGMCSFSandwich6,000 - 8.23 (pg/mL)4,200 (pg/mL)10.7 (pg/mL)4.12 (pg/mL)11%7%95%IFNalphaSandwich400 - 0.55 (pg/mL)280 (pg/mL)0.72 (pg/mL)0.27 (pg/mL)9%5%100%IFNgammaSandwich99 - 0.14 (pg/mL)69.3 (pg/mL)0.18 (pg/mL)0.068 (pg/mL)10%5%99%IL-1betaSandwich4,075 - 5.59 (pg/mL)2,852 (pg/mL)7.27 (pg/mL)2.79 (pg/mL)11%4%103%IL-1RASandwich7,000 - 9.6 (pg/mL)4,900 (pg/mL)12.48 (pg/mL)4.8 (pg/mL)11%7%102%IL-2Sandwich64 - 0.088 (pg/mL)44.8 (pg/mL)0.11 (pg/mL)0.044 (pg/mL)8%6%101%IL-2RalphaSandwich16,000 - 21.95 (pg/mL)11,200 (pg/mL)28.5 (pg/mL)10.97 (pg/mL)UK9%101%IL-6Sandwich333 - 0.46 (pg/mL)233 (pg/mL)0.59 (pg/mL)0.23 (pg/mL)8%6%91%IL-6RSandwich1,250 - 1.71 (pg/mL)291 (pg/mL)2.23 (pg/mL)0.86 (pg/mL)UK6.5%111%IL-8Sandwich2,000 - 2.74 (pg/mL)1,400 (pg/mL)3.6 (pg/mL)1.37 (pg/mL)12%8%100%IL-10Sandwich2,680 - 3.68 (pg/mL)1,876 (pg/mL)4.78 (pg/mL)1.84 (pg/mL)11%3%99%IL-12p70Sandwich3,000 - 4.12 (pg/mL)2,100 (pg/mL)5.35 (pg/mL)2.06 (pg/mL)12%10%99%IL-13Sandwich333 - 0.46 (pg/mL)233 (pg/mL)0.59 (pg/mL)0.23 (pg/mL)11%5%105%MCP-1Sandwich2,000 - 2.74 (pg/mL)467 (pg/mL)3.6 (pg/mL)1.37 (pg/mL)9%4%99%MIP-1alphaSandwich2,000 - 2.74 (pg/mL)467 (pg/mL)3.57 (pg/mL)1.37 (pg/mL)UK4%114%TNFalphaSandwich661 - 0.91 (pg/mL)462 (pg/mL)1.18 (pg/mL)0.45 (pg/mL)12%8%101%

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. Reagents include: