E. coli-derived human RAB27A recombinant protein (Position: L98-K216).
Alternative Names:
GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A
Boster Bio Anti-RAB27A Antibody Picoband (monoclonal, 2F5) catalog M01608. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Monoclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
IHC analysis of RAB27A using anti-RAB27A antibody (M01608).RAB27A was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1021) with DAB as the chromogen.
IHC analysis of RAB27A using anti-RAB27A antibody (M01608).RAB27A was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1021) with DAB as the chromogen.
IHC analysis of RAB27A using anti-RAB27A antibody (M01608).RAB27A was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1021) with DAB as the chromogen.
IHC analysis of RAB27A using anti-RAB27A antibody (M01608).RAB27A was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1021) with DAB as the chromogen.
IHC analysis of RAB27A using anti-RAB27A antibody (M01608).RAB27A was detected in paraffin-embe
Western blot analysis of RAB27A using anti-RAB27A antibody (M01608). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates Lane 2: human HepG2 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human HT1080 whole cell lysates Lane 5: human SW620 whole cell lysates Lane 6: human PANC-1 whole cell lysates Lane 7: rat RH35 whole cell lysates Lane 8: mouse NIH/3T3 whole cell lysates After Electrophoresis&44, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RAB27A antigen affinity purified monoclonal antibody (Catalog M01608) at 0.5 µg/mL overnight at 4C&44, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1001) with Tanon 5200 system. A specific band was detected for RAB27A at approximately 27KD. The expected band size for RAB27A is at 27KD.
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