PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation me
Application Dilute:
WB 1:2000IHC 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Cross-reactivity test with ZEB2: HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right) and immunoblotted with anti-ZEB1 (M00548-1).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZEB1 monoclonal antibody at 1:200 dilution. (HepG2: human, HeLa: human, SVT2: mouse, A549: human, COS7: monkey, Jurkat: human, MDCK: canine, PC12: rat, MCF7: human)
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