E.coli-derived human NDUFB10 recombinant protein (Position: D36-S172).
Alternative Names:
CI PDSW, Complex I PDSW, NDUFB10, PDSW
Boster Bio Anti-NDUFB10 Antibody Picoband catalog A11886-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.1-0.25µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human ELISA, 0.1-0.5µg/ml, -
IHC analysis of NDUFB10 using anti-NDUFB10 antibody (A11886-3). NDUFB10 was detected in paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-NDUFB10 Antibody (A11886-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of NDUFB10 using anti-NDUFB10 antibody (A11886-3). NDUFB10 was detected in paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-NDUFB10 Antibody (A11886-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of NDUFB10 using anti-NDUFB10 antibody (A11886-3). NDUFB10 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-NDUFB10 Antibody (A11886-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of NDUFB10 using anti-NDUFB10 antibody (A11886-3). NDUFB10 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-NDUFB10 Antibody (A11886-3) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of NDU
Western blot analysis of NDUFB10 using anti-NDUFB10 antibody (A11886-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human HL-60 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB10 antigen affinity purified polyclonal antibody (Catalog A11886-3) at 0.25 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for NDUFB10 at approximately 22KD. The expected band size for NDUFB10 is at 22KD.
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