A synthetic peptide corresponding to a sequence in the middle region of human HOXC4, which shares 95.7% amino acid (aa) sequence identity with mouse and rat HOXC4.
Alternative Names:
cp19, homeobox C4, Homeobox protein CP19, Homeobox protein Hox 3E, Homeobox protein Hox C4, HOX3, HOX3E, HOXC4
Boster Bio Anti-HOXC4 Antibody Picoband catalog A09869-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.25-0.5 µg/ml/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 µg/ml/1x10 6 cells, Human, Rat
IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.
Western blot analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXC4 antigen affinity purified polyclonal antibody (Catalog A09869-1) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for HOXC4 at approximately 36 kDa. The expected band size for HOXC4 is at 36 kDa.
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